The existence of chemical species inside biological material is identified and confirmed using a Double Beam UV Spectrophotometer system. The diffraction mechanism separates a single beam of light into two beams. For the spectrometry procedure, the monochromatic beam picks a certain wavelength of light.
UV Spectrometers are used in a wide range of scientific fields, including material sciences, biochemistry, molecular biology, physics, and chemistry. These devices are used in industries that deal with semiconductors, optical manufacturing, lasers, printing, forensic exams, as well as laboratory studies. For spectroscopic analysis, the equipment quantifies various analyses such as chemical substances and biological macromolecules. The majority of spectroscopic investigation is done on solutions; however compounds in solid and gracious forms are also investigated.
Difference between a Single Beam and Double Beam Spectrophotometer
| Single Beam Spectrophotometer | Double Beam Spectrophotometer |
| Light waves travel through the sample in a single beam Spectrophotometer. | However, in a double beam Spectrophotometer, the light waves divide into two sections, enabling just one split component of the light beam to pass through the sample. |
| Single beam spectrophotometers are more compact and optically simpler. These are lower-cost items | Double beam are expensive as compared to single beam, and a little complex in structure. |
| Light hits through a single source in a single beam spectrophotometer. | The light source is used in the double beam spectrophotometer, and the transmittance is separated into two portions. |
Benefits of Single Beam Design
- Because there are fewer optical components, light transmission and noise are enhanced.
- Higher light throughput allows for increased sensitivity.
Benefits of Double Beam Design
- Absorbance correction for solvent blank
- There may be variations in light energy between ratio recording measures in a single beam configuration.
- In real-time, corrections for stray light, beam intensity changes, and electronic noise are implemented.
The advantages of a twin beam system considerably surpass the advantages of single-beam systems in terms of sensitivity. Double beam systems are becoming more popular due to recent breakthroughs in optics and electronics.
Why select the iGene Labserve Double Beam UV spectrophotometer?
The iGeneLabserve Double Beam UV spectrophotometer System has Auto wavelength selection and Individual control over D2 and Visible lamps, which are designed to last a lifetime and are easy to replace. Cuvettes of varying route lengths can be accommodated in the large sample container. Learn more about the characteristics and benefits of the product before you buy it at https://www.igenels.com/ or call 09310696848 for more information directly.